The calculation of transcript flux ratios reveals single regulatory mechanisms capable of activation and repression.

ABSTRACT: The regulation of transcription allows cells to adjust the rate of RNA polymerases (RNAPs) initiated in a promoter-specific manner. Classically, transcription factors are directed to a subset of promoters via the recognition of DNA sequence motifs. However, a unique class of regulators is recruited directly through interactions with RNAP. Surprisingly, these factors may still possess promoter specificity, and it has been postulated that the same kinetic mechanism leads to different regulatory outcomes depending on a promoter's basal rate constants. However, mechanistic studies of regulation typically report factor activity in terms of changes in the thermodynamics or kinetics of individual steps or states while qualitatively linking these observations to measured changes in transcript prod

SUBMITTER: Galburt EA 

PROVIDER: S-EPMC6294943 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature