Intra-epidemic genome variation in highly pathogenic African swine fever virus (ASFV) from the country of Georgia.

ABSTRACT:

Background

African swine fever virus (ASFV) causes an acute hemorrhagic infection in suids with a mortality rate of up to 100%. No vaccine is available and the potential for catastrophic disease in Europe remains elevated due to the ongoing ASF epidemic in Russia and Baltic countries. To date, intra-epidemic whole-genome variation for ASFV has not been reported. To provide a more comprehensive baseline for genetic variation early in the ASF outbreak, we sequenced two Georgian ASFV samples, G-2008/1 and G-2008/2, derived from domestic porcine blood collected in 2008.

Methods

Genomic DNA was extracted directly from low-volume ASFV PCR-positive porcine blood samples and subjected to next generation sequencing on the Illumina Miseq platform. De novo and mapped sequence assemblie

SUBMITTER: Farlow J 

PROVIDER: S-EPMC6295034 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature