LncRNA H19 promotes the proliferation of pulmonary artery smooth muscle cells through AT₁R via sponging let-7b in monocrotaline-induced pulmonary arterial hypertension.

ABSTRACT:

Background

Pulmonary arterial hypertension (PAH) is related to inflammation, and the lncRNA H19 is associated with inflammation. However, whether PDGF-BB-H19-let-7b-AT₁R axis contributes to the pathogenesis of PAH has not been thoroughly elucidated to date. This study investigated the role of H19 in PAH and its related mechanism.

Methods

In the present study, SD rats, C57/BL6 mice and H19-/- mice were injected with monocrotaline (MCT) to establish a PAH model. H19 was detected in the cytokine-stimulated pulmonary arterial smooth muscle cells (PASMCs), serum and lungs of rats/mice. H19 overexpression and knockdown experiments were also conducted. A dual luciferase reporter assay was used to explore whether let-7b is a sponge miRNA of H19, and AT₁R is a n

SUBMITTER: Su H 

PROVIDER: S-EPMC6295077 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature