Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding.

ABSTRACT:

Background

DNA metabarcoding, commonly used in exploratory microbial ecology studies, is a promising method for the simultaneous in planta-detection of multiple pathogens associated with disease complexes, such as the grapevine trunk diseases. Profiling of pathogen communities associated with grapevine trunk diseases is particularly challenging, due to the presence within an individual wood lesion of multiple co-infecting trunk pathogens and other wood-colonizing fungi, which span a broad range of taxa in the fungal kingdom. As such, we designed metabarcoding primers, using as template the ribosomal internal transcribed spacer of grapevine trunk-associated ascomycete fungi (GTAA) and compared them to two universal primer widely used in microbial ecology.

Results

We first per

SUBMITTER: Morales-Cruz A 

PROVIDER: S-EPMC6295080 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature