Seselin ameliorates inflammation via targeting Jak2 to suppress the proinflammatory phenotype of macrophages.

ABSTRACT:

Background and purpose

Sepsis is a serious clinical condition with a high mortality rate. Anti inflammatory agents have been found to be beneficial for the treatment of sepsis. Here, we have evaluated the anti-inflammatory activity of seselin in models of sepsis and investigated the underlying molecular mechanism(s).

Experimental approach

In vivo therapeutic effects of seselin was evaluated in two models of sepsis, caecal ligation and puncture or injection of LPS, in C57BL/6 mice. In vitro, anti-inflammatory activity of seselin was assessed with macrophages stimulated with LPS and IFN-γ. Anti inflammatory actions were analysed with immunohistochemical methods, ELISA and Western blotting. Flow cytometry was used to assess markers of macrophage phenotype (pro- or anti-inflamma

SUBMITTER: Feng L 

PROVIDER: S-EPMC6295420 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature