Immunogenicity of a novel tetravalent dengue envelope protein domain III-based antigen in mice.

ABSTRACT: Dengue virus is a mosquito-borne pathogen that causes dengue diseases. All four serotypes of dengue virus are infectious for humans. Therefore, an efficacious dengue vaccine should be tetravalent to provide protection against all types of virus. The goal of this study was to design a new tetravalent recombinant protein from envelope protein of dengue viruses to induce virus-neutralizing antibodies against all four serotypes in mice. A chimeric protein was designed from domain III of envelope protein of all serotypes of dengue virus. Four domain III fragments were linked together by alpha helix making linkers. The final sequence of the designed protein was analyzed in silico and the coding gene sequence was deduced by reverse translation. After cloning and expression of the recombinant prot

SUBMITTER: Fahimi H 

PROVIDER: S-EPMC6295631 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature