Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity.

ABSTRACT: An increase in oxidative protein damage is a leading contributor to the age-associated decline in oocyte quality. By removing such damaged proteins, the proteasome plays an essential role in maintaining the fidelity of oocyte meiosis. In this study, we established that decreased proteasome activity in naturally aged, germinal vesicle (GV) mouse oocytes positively correlates with increased protein modification by the lipid aldehyde 4-hydroxynonenal (4-HNE). Furthermore, attenuation of proteasome activity in GV oocytes of young animals was accompanied by an increase in 4-HNE-modified proteins, including α-tubulin, thereby contributing to a reduction in tubulin polymerization, microtubule stability, and integrity of oocyte meiosis. A decrease in proteasome activity was also recapitulated in t

SUBMITTER: Mihalas BP 

PROVIDER: S-EPMC6295717 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature