Rapid and low-cost multiplex synthesis of chemokine analogs.

ABSTRACT: Peptides represent a promising source of new medicines, but improved technologies are needed to facilitate discovery and optimization campaigns. In particular, longer peptides with multiple disulfide bridges are challenging to produce, and producing large numbers of structurally related variants is dissuasively costly and time-consuming. The principal cost and time drivers are the multiple column chromatography purification steps that are used during the multistep chemical synthesis procedure, which involves both ligation and oxidative refolding steps. In this study, we developed a method for multiplex parallel synthesis of complex peptide analogs in which the structurally variant region of the molecule is produced as a small peptide on a 384-well synthesizer with subsequent ligation to th

SUBMITTER: Paolini-Bertrand M 

PROVIDER: S-EPMC6295741 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature