Differential parameters between cytosolic 2-Cys peroxiredoxins, PRDX1 and PRDX2.

ABSTRACT: Peroxiredoxins are thiol-dependent peroxidases that function in peroxide detoxification and H₂ O₂ induced signaling. Among the six isoforms expressed in humans, PRDX1 and PRDX2 share 97% sequence similarity, 77% sequence identity including the active site, subcellular localization (cytosolic) but they hold different biological functions albeit associated with their peroxidase activity. Using recombinant human PRDX1 and PRDX2, the kinetics of oxidation and hyperoxidation with H₂ O₂ and peroxynitrite were followed by intrinsic fluorescence. At pH 7.4, the peroxidatic cysteine of both isoforms reacts nearly tenfold faster with H₂ O₂ than with peroxynitrite, and both reactions are orders of magnitude faster than with most protei

SUBMITTER: Dalla Rizza J 

PROVIDER: S-EPMC6295901 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature