Genetic Analysis of Toxocara Spp. in Stray Cats and Dogs in Van Province, Eastern Turkey.

ABSTRACT:

Introduction

Toxocara canis and Toxocara cati are roundworms of dogs and cats. The purpose of this study was to investigate the infection caused by these ascarids in cats and dogs, using microscopic and molecular analysis methods.

Material and methods

Adult ascarids were gathered from the faeces of dogs and cats in Van province, in 2015-2016. Existing keys and PCR sequencing of the ITS-2 fragment were used to identify the morphological features of the parasite species.

Results

It was observed that out of 20 adult ascarids, 17 and 3 were found to be Toxocara canis and Toxocara cati, respectively. The ITS-2 gene region was amplified by PCR to perform molecular analysis. Genotyping indicated that the dogs and cats were infected with T. cani

SUBMITTER: Oguz B 

PROVIDER: S-EPMC6296006 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature