Conversion of human adipose-derived stem cells into functional and expandable endothelial-like cells for cell-based therapies.

ABSTRACT:

Background

Ischemic vascular diseases are the major cause of death worldwide. In recent years, endothelial cell (EC)-based approaches to vascular regeneration are increasingly viable strategies for treating ischemic diseases, but their applications are challenged by the difficulties in their efficient generation and stable maintenance. Here, we show an alternative protocol that facilitates the generation of functional and expandable ETS variant 2 (ETV2)-induced endothelial-like cells (EiECs) from human adipose-derived stem cells (hADSCs), providing a potential source of cells for autologous ECs to treat ischemic vascular diseases.

Methods

hADSCs were obtained from fresh human adipose tissue. Passage 3 hADSCs were transduced with doxycycline (DOX)-inducible ETV2 transcription

SUBMITTER: Cheng F 

PROVIDER: S-EPMC6296081 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature