Morphometric analysis and neuroanatomical mapping of the zebrafish brain.

ABSTRACT: Large-scale genomic studies have recently identified genetic variants causative for major neurodevelopmental disorders, such as intellectual disability and autism. However, determining how underlying developmental processes are affected by these mutations remains a significant challenge in the field. Zebrafish is an established model system in developmental neurogenetics that may be useful in uncovering the mechanisms of these mutations. Here we describe the use of voxel-intensity, deformation field, and volume-based morphometric techniques for the systematic and unbiased analysis of gene knock-down and environmental exposure-induced phenotypes in zebrafish. We first present a computational method for brain segmentation based on transgene expression patterns to create a comprehensive neuro

SUBMITTER: Gupta T 

PROVIDER: S-EPMC6296225 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature