Single sample sequencing (S3EQ) of epigenome and transcriptome in nucleus accumbens.

ABSTRACT:

Background

High-throughput sequencing has been widely applied to uncover the molecular mechanisms underlying neurological and psychiatric disorders. The large body of data support the role of epigenetic mechanisms in neurological function of both human and animals. Yet, the existing data is limited by the fact that epigenetic and transcriptomic changes have only been measured in separate cohorts. This has limited precise correlation of epigenetic changes in gene expression.

New method

Single Sample Sequencing (S3EQ) is an innovative approach to analyze both epigenetic and transcriptomic regulation within a single neuronal sample. Using this method, we analyzed chromatin immunoprecipitation (ChIP)- and RNA-sequencing data from the nucleus accumbens (NAc) of the same animal.

SUBMITTER: Xu SJ 

PROVIDER: S-EPMC6296235 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature