Human monoclonal antibody fragments targeting matrilin-3 in growth plate cartilage.

ABSTRACT: PURPOSE:Many genetic disorders, including chondrodysplasias, and acquired disorders impair growth plate function, resulting in short and sometimes malformed bones. There are multiple endocrine and paracrine factors that promote chondrogenesis at the growth plate, which could potentially be used to treat these disorders. Targeting these growth factors specifically to the growth plate might augment the therapeutic skeletal effect while diminishing undesirable effects on non-target tissues. METHODS:Using yeast display technology, we selected single-chain variable antibody fragments that bound to human and mouse matrilin-3, an extracellular matrix protein specifically expressed in cartilage tissue. The ability of the selected antibody fragments to bind matrilin-3 and to bind cartilage tissue i

SUBMITTER: Cheung CS 

PROVIDER: S-EPMC6296366 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature