Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury.

ABSTRACT: Engineered skeletal muscle tissues can be used for in vitro studies that require physiologically relevant models of native tissues. Herein, we describe the development of a three-dimensional (3D) skeletal muscle tissue that recapitulates the architectural and structural complexities of muscle within a microfluidic device. Using a 3D photo-patterning approach, we spatially confined a cell-laden gelatin network around two bio-inert hydrogel pillars, which induce uniaxial alignment of the cells and serve as anchoring sites for the encapsulated cells and muscle tissues as they form and mature. We have characterized the tissue morphology and strain profile during differentiation of the cells and skeletal muscle tissue formation by using a combination of fluorescence microscopy and computational

SUBMITTER: Agrawal G 

PROVIDER: S-EPMC6296378 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature