Incorporation of macrophages into engineered skeletal muscle enables enhanced muscle regeneration.

ABSTRACT: Adult skeletal muscle has a robust capacity for self-repair, owing to synergies between muscle satellite cells and the immune system. In vitro models of muscle self-repair would facilitate the basic understanding of muscle regeneration and the screening of therapies for muscle disease. Here, we show that the incorporation of macrophages into muscle tissues engineered from adult-rat myogenic cells enables near-complete structural and functional repair after cardiotoxic injury in vitro. First, we show that-in contrast with injured neonatal-derived engineered muscle-adult-derived engineered muscle fails to properly self-repair after injury, even when treated with pro-regenerative cytokines. We then show that rat bone-marrow-derived macrophages or human blood-derived macrophages resident withi

SUBMITTER: Juhas M 

PROVIDER: S-EPMC6296488 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature