Strep-tag II fusion technology for the modification and immobilization of lipase B from Candida antarctica (CALB).

ABSTRACT: Fusion tags - amino acid sequences that are genetically coded to be expressed as attached moieties to a protein - have the potential to enhance the activity of native enzyme, enable specific purification of the enzyme, and promote simple and efficient immobilization of enzymes onto material supports. In this work, we demonstrate the effect of a Strep-tag II fusion tag on the properties of free and immobilized lipase B from Candida antarctica (CALB). The gene encoding the mature portion of CALB was codon-optimized and cloned in pASG-IBA2 plasmid for expression in E. coli. Purified recombinant Strep-tag II CALB was immobilized to Strep-Tactin based support through affinity binding, and the immobilized and free Strep-tag II CALB were compared to a commercial CALB. Following modi

SUBMITTER: Johar SS 

PROVIDER: S-EPMC6296563 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature