Construction and sequencing analysis of scFv antibody fragment derived from monoclonal antibody against norfloxacin (Nor155).

ABSTRACT: Norfloxacin belongs to the group of fluoroquinolone antibiotics which has been approved for treatment in animals. However, its residues in animal products can pose adverse side effects to consumer. Therefore, detection of the residue in different food matrices must be concerned. In this study, a single chain variable fragment (scFv) that recognizes norfloxacin antibiotic was constructed. The cDNA was synthesized from total RNA of hybridoma cells against norfloxacin. Genes encoding V_H and V_L regions of monoclonal antibody against norfloxacin (Nor155) were amplified and size of V_H and V_L fragments was 402 bp and 363 bp, respectively. The scFv of Nor155 was constructed by an addition of (Gly₄Ser)₃ as a linker between V_H

SUBMITTER: Mala J 

PROVIDER: S-EPMC6296615 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature