Polymerization and depolymerization of actin with nucleotide states at filament ends.

ABSTRACT: Polymerization induces hydrolysis of ATP bound to actin, followed by γ-phosphate release, which helps advance the disassembly of actin filaments into ADP-G-actin. Mechanical understanding of this correlation between actin assembly and ATP hydrolysis has been an object of intensive studies in biochemistry and structural biology for many decades. Although actin polymerization and depolymerization occur only at either the barbed or pointed ends and the kinetic and equilibrium properties are substantially different from each other, characterizing their properties is difficult to do by bulk assays, as these assays report the average of all actin filaments in solution and are therefore not able to discern the properties of individual actin filaments. Biochemical studies of actin polymerization a

SUBMITTER: Fujiwara I 

PROVIDER: S-EPMC6297080 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature