Reference genes identification for normalization of qPCR under multiple stresses in Hordeum brevisubulatum.

ABSTRACT:

Background

Real-time quantitative PCR has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. Wild barley (Hordeum brevisubulatum (Trin.) Link) is a wild relative species in Triticeae that has strong tolerance to abiotic stresses and extremely wide adaptation. However, suitable references gene have not been documented for standardization of gene expression in wild barley under abiotic stress.

Results

Here we report the first systematic and comprehensive analysis of reference genes for quantitative real-time PCR standardization in wild barley. We selected 11 genes, including ACT (Actin), ADP (ADP-ribosylation factor 1), CYP2 (Cyclophilin 2), EF-1α</

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6297944 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature