A descriptive marker gene approach to single-cell pseudotime inference.

ABSTRACT:

Motivation

Pseudotime estimation from single-cell gene expression data allows the recovery of temporal information from otherwise static profiles of individual cells. Conventional pseudotime inference methods emphasize an unsupervised transcriptome-wide approach and use retrospective analysis to evaluate the behaviour of individual genes. However, the resulting trajectories can only be understood in terms of abstract geometric structures and not in terms of interpretable models of gene behaviour.

Results

Here we introduce an orthogonal Bayesian approach termed 'Ouija' that learns pseudotimes from a small set of marker genes that might ordinarily be used to retrospectively confirm the accuracy of unsupervised pseudotime algorithms. Crucially, we model these genes in terms of

SUBMITTER: Campbell KR 

PROVIDER: S-EPMC6298060 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature