The rectal cancer microRNAome--microRNA expression in rectal cancer and matched normal mucosa.

ABSTRACT:

Purpose

miRNAs play a prominent role in a variety of physiologic and pathologic biologic processes, including cancer. For rectal cancers, only limited data are available on miRNA expression profiles, whereas the underlying genomic and transcriptomic aberrations have been firmly established. We therefore, aimed to comprehensively map the miRNA expression patterns of this disease.

Experimental design

Tumor biopsies and corresponding matched mucosa samples were prospectively collected from 57 patients with locally advanced rectal cancers. Total RNA was extracted, and tumor and mucosa miRNA expression profiles were subsequently established for all patients. The expression of selected miRNAs was validated using semi-quantitative real-time PCR.

Results

Forty-nine miRNAs wer

SUBMITTER: Gaedcke J 

PROVIDER: S-EPMC6298229 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature