Quantification of Platelet Contractile Movements during Thrombus Formation.

ABSTRACT: Imaging methods based on time-lapse microscopy are important tools for studying the dynamic events that shape thrombus formation upon vascular injury. However, there is a lack of methods to translate the vast amount of visual data generated in such experiments into quantitative variables describing platelet movements that can be subjected to systematic analysis. In this study, we developed experimental and computational protocols allowing for a detailed mathematical analysis of platelet movements within a developing thrombus. We used a flow chamber-based model of thrombosis wherein a collagen strip was used to initiate platelet adhesion and activation. Combining the use of a platelet staining protocol, designed to enable identification of individual platelets, and image processing, we trac

SUBMITTER: Tunstromer K 

PROVIDER: S-EPMC6298232 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature