KILchip v1.0: A Novel <i>Plasmodium falciparum</i> Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization.

ABSTRACT: Passive transfer studies in humans clearly demonstrated the protective role of IgG antibodies against malaria. Identifying the precise parasite antigens that mediate immunity is essential for vaccine design, but has proved difficult. Completion of the Plasmodium falciparum genome revealed thousands of potential vaccine candidates, but a significant bottleneck remains in their validation and prioritization for further evaluation in clinical trials. Focusing initially on the Plasmodium falciparum merozoite proteome, we used peer-reviewed publications, multiple proteomic and bioinformatic approaches, to select and prioritize potential immune targets. We expressed 109 P. falciparum recombinant proteins, the majority of which were obtained using a mammalian expression syste

SUBMITTER: Kamuyu G 

PROVIDER: S-EPMC6298441 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature