Proteomic Profiling Exosomes from Vascular Smooth Muscle Cell.

ABSTRACT: PURPOSE:Vascular smooth muscle cells (VSMC) and endothelial cells (EC) communicate mutually to coordinate vascular development and homeostasis. Exosomes are emerging as one type of the mediators involved in this communication. Characterizing proteins in the exosomes is the critical first step in understanding how the VSMC-EC crosstalk is mediated by exosomes. EXPERIMENTAL DESIGN:The proteins in the human VSMC-derived exosomes are profiled using nanoLC-MS/MS based proteomics. The identified proteins are subjected to gene ontology analysis. The VSMC-derived exosomes are also assessed for proangiogenic activity in vivo. RESULTS:Four hundred and fifty-nine proteins are identified in the VSMC-derived exosomes. Gene ontology analysis revealed that the exosome proteins are involved in 179 cellula

SUBMITTER: Qiu H 

PROVIDER: S-EPMC6298740 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature