Mass Spectrometry-Based Chemical Cartography of a Cardiac Parasitic Infection.

ABSTRACT: Trypanosoma cruzi parasites are the causative agents of Chagas disease, a leading infectious form of heart failure whose pathogenesis is still not fully characterized. In this work, we applied untargeted liquid chromatography-tandem mass spectrometry to heart sections from T. cruzi-infected and uninfected mice. We combined molecular networking and three-dimensional modeling to generate chemical cartographical heart models. This approach revealed for the first time preferential parasite localization to the base of the heart and regiospecific distributions of nucleoside derivatives and eicosanoids, which we correlated to tissue-damaging immune responses. We further detected novel cardiac chemical signatures related to the severity and ultimate outcome of the infection. These signatures inclu

SUBMITTER: McCall LI 

PROVIDER: S-EPMC6298790 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature