In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone.

ABSTRACT: Osteoblasts are matrix-depositing cells that can divide and heal bone injuries. Their deep-tissue location and the slow progression of bone regeneration challenge attempts to capture osteoblast behaviors in live tissue at high spatiotemporal resolution. Here, we have developed an imaging platform to monitor and quantify individual and collective behaviors of osteoblasts in adult zebrafish scales, skeletal body armor discs that regenerate rapidly after loss. Using a panel of transgenic lines that visualize and manipulate osteoblasts, we find that a founder pool of osteoblasts emerges through de novo differentiation within one day of scale plucking. These osteoblasts undergo division events that are largely uniform in frequency and orientation to establish a primordium. Osteoblast proliferat

SUBMITTER: Cox BD 

PROVIDER: S-EPMC6298855 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature