P2Y₂ and P2Y₆ receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells.

ABSTRACT: Adipose tissue contains self-renewing multipotent cells termed mesenchymal stromal cells. In situ, these cells serve to expand adipose tissue by adipogenesis, but their multipotency has gained interest for use in tissue regeneration. Little is known regarding the repertoire of receptors expressed by adipose-derived mesenchymal stromal cells (AD-MSCs). The purpose of this study was to undertake a comprehensive analysis of purinergic receptor expression. Mesenchymal stromal cells were isolated from human subcutaneous adipose tissue and confirmed by flow cytometry. The expression profile of purinergic receptors was determined by quantitative real-time PCR and immunocytochemistry. The molecular basis for adenine and uracil nucleotide-evoked intracellular calcium responses was determined using

SUBMITTER: Ali S 

PROVIDER: S-EPMC6298923 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature