Uncovering secondary metabolite evolution and biosynthesis using gene cluster networks and genetic dereplication.

ABSTRACT: The increased interest in secondary metabolites (SMs) has driven a number of genome sequencing projects to elucidate their biosynthetic pathways. As a result, studies revealed that the number of secondary metabolite gene clusters (SMGCs) greatly outnumbers detected compounds, challenging current methods to dereplicate and categorize this amount of gene clusters on a larger scale. Here, we present an automated workflow for the genetic dereplication and analysis of secondary metabolism genes in fungi. Focusing on the secondary metabolite rich genus Aspergillus, we categorize SMGCs across genomes into SMGC families using network analysis. Our method elucidates the diversity and dynamics of secondary metabolism in section Nigri, showing that SMGC diversity within the section has the same magni

SUBMITTER: Theobald S 

PROVIDER: S-EPMC6298953 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature