Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks.

ABSTRACT: Collective cell migration mediates multiple tissue morphogenesis processes. Yet how multi-dimensional mesenchymal cell movements are coordinated remains mostly unknown. Here we report that coordinated mesenchymal cell migration during chicken feather elongation is accompanied by dynamic changes of bioelectric currents. Transcriptome profiling and functional assays implicate contributions from functional voltage-gated Ca²⁺ channels (VGCCs), Connexin-43 based gap junctions, and Ca²⁺ release activated Ca²⁺ (CRAC) channels. 4-Dimensional Ca²⁺ imaging reveals that the Sonic hedgehog-responsive mesenchymal cells display synchronized Ca²⁺ oscillations, which expand progressively in area during feather elongation. Inhibiting VGCCs, gap junctio

SUBMITTER: Li A 

PROVIDER: S-EPMC6299091 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature