Exploring the rumen fluid metabolome using liquid chromatography-high-resolution mass spectrometry and Molecular Networking.

ABSTRACT: The rumen primary and secondary metabolite content is intimately related to its community of bacteria, protozoa, fungi, archaea and bacteriophages, ingested feed and the host. Despite the myriad of interactions and novel compounds to be discovered, few studies have explored the rumen metabolome. Here, we present the first study using ultra-high performance liquid chromatography tandem mass-spectrometry and Molecular Networking approach, and various extraction methods on the cell-free rumen fluid of a non-lactating Holstein cow. Putative molecules were annotated based on accurate fragmentation matching the Global Natural Products Social Molecular Networking library, public spectral libraries, or annotated manually. The combination of five extraction methods resulted on 1,882 molecular featu

SUBMITTER: de Almeida RTR 

PROVIDER: S-EPMC6299289 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature