Delivery of miR-146a to Ly6C^high Monocytes Inhibits Pathogenic Bone Erosion in Inflammatory Arthritis.

ABSTRACT: Rationale: Monocytes play critical roles in the pathogenesis of arthritis by contributing to the inflammatory response and bone erosion. Among genes involved in regulating monocyte functions, miR-146a negatively regulates the inflammatory response and osteoclast differentiation of monocytes. It is also the only miRNA reported to differentially regulate the cytokine response of the two classical Ly6C^high and non-classical Ly6C^low monocyte subsets upon bacterial challenge. Although miR-146a is overexpressed in many tissues of arthritic patients, its specific role in monocyte subsets under arthritic conditions remains to be explored. Methods: We analyzed the monocyte subsets during collagen-induced arthritis (CIA) development by flow cytometry. We quantifie

SUBMITTER: Ammari M 

PROVIDER: S-EPMC6299444 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature