Cow dung is an ideal fermentation medium for amylase production in solid-state fermentation by Bacillus cereus.

ABSTRACT: Amylase production by Bacillus cereus IND4 was investigated by solid state fermentation (SSF) using cow dung substrate. The SSF conditions were optimized by using one-variable-at-a-time approach and two level full factorial design. Two level full factorial design demonstrated that moisture, pH, fructose, yeast extract and ammonium sulphate have significantly influenced enzyme production (p < 0.05). A central composite design was employed to investigate the optimum concentration of these variables affecting amylase production. Maximal amylase production of 464 units/ml of enzyme was observed in the presence of 100% moisture, 0.1% fructose and 0.01% ammonium sulphate. The enzyme production increased three fold compared to the original medium. The optimum pH and temperature for

SUBMITTER: Vijayaraghavan P 

PROVIDER: S-EPMC6299861 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature