Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matter.

ABSTRACT: The study investigated the biochemical characteristics and kinetic parameters of laccase from a newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decay plant litters. This was with a view to identifying the type of laccase and its possible suitability for biotechnological applications. The fungal strain was identified as A. pullulans NAC8 by sequencing of its 5.8S rRNA and adjacent internally transcribed sequences (ITS) 1 and 2. A. pullulans NAC8 laccase was purified 2.0-fold with a yield of 59.3% and specific activity of 9.34 μmol/min/mg protein. The kinetic parameters K_M , V _max, k _cat and k _cat/K_M for laccase with guaiacol as substrate were 1.05 ± 0.12 m

SUBMITTER: Ademakinwa AN 

PROVIDER: S-EPMC6299892 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature