A pilot study evaluating the utility of commercially available antibodies for flow cytometric analysis of Panthera species lymphocytes.

ABSTRACT:

Background

The immune response against tuberculosis in lions is still poorly defined and our understanding is hampered by the lack of lion specific reagents. The process for producing antibodies against a specific antigen is laborious and not available to many research laboratories. As the search for antibody cross-reactivity is an important strategy for immunological studies in veterinary medicine, we have investigated the use of commercially available antibodies to characterize T cell subsets in African lions (Panthera leo).

Results

Commercially available antibodies were screened and investigated the influence of two different sample processing methods, as well as the effect of time delay on cell surface marker expression on lion lymphocytes. Using commercially available a

SUBMITTER: Sylvester TT 

PROVIDER: S-EPMC6299994 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature