The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation.

ABSTRACT:

Background

Microglial inflammatory activation is the common feature of the central nervous system (CNS) diseases. Microglia can be activated and particularly polarized toward a dual role in the injured CNS. The CD200 receptor 1 (CD200R1) inhibits inflammatory microglia activation as illustrated by studies. Publications show abnormal activation of microglia secondary to the deficient inhibit of CD200-CD200R interaction. In the present study, we established a neuronal-microglia co-culture system to investigate the association between CD200R1 engagement and classical microglial activation. We analyzed the glycosylation of CD200R1 and the CD200 binding. Secretion of pro-inflammatory cytokines were measured.

Results

CD200R1 was N-glycosylated at Asparagine 44 (Asn44, N44). Mutati

SUBMITTER: Liu C 

PROVIDER: S-EPMC6300008 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature