In vivo monitoring of intracellular Ca²⁺ dynamics in the pancreatic β-cells of zebrafish embryos.

ABSTRACT: Assessing the response of pancreatic islet cells to glucose stimulation is important for understanding β-cell function. Zebrafish are a promising model for studies of metabolism in general, including stimulus-secretion coupling in the pancreas. We used transgenic zebrafish embryos expressing a genetically-encoded Ca²⁺ sensor in pancreatic β-cells to monitor a key step in glucose induced insulin secretion; the elevations of intracellular [Ca²⁺]_i. In vivo and ex vivo analyses of [Ca²⁺]_i demonstrate that β-cell responsiveness to glucose is well established in late embryogenesis and that embryonic β-cells also respond to free fatty acid and amino acid challenges. In vivo imaging of whole embryos further shows that indirect glucose administ

SUBMITTER: Lorincz R 

PROVIDER: S-EPMC6300091 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature