ORAI1 Activates Proliferation of Lymphatic Endothelial Cells in Response to Laminar Flow Through Kruppel-Like Factors 2 and 4.

ABSTRACT:

Rationale

Lymphatic vessels function to drain interstitial fluid from a variety of tissues. Although shear stress generated by fluid flow is known to trigger lymphatic expansion and remodeling, the molecular basis underlying flow-induced lymphatic growth is unknown.

Objective

We aimed to gain a better understanding of the mechanism by which laminar shear stress activates lymphatic proliferation.

Methods and results

Primary endothelial cells from dermal blood and lymphatic vessels (blood vascular endothelial cells and lymphatic endothelial cells [LECs]) were exposed to low-rate steady laminar flow. Shear stress-induced molecular and cellular responses were defined and verified using various mutant mouse models. Steady laminar flow induced the classic shear stress respo

SUBMITTER: Choi D 

PROVIDER: S-EPMC6300148 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature