Comparison of EpCAM^highCD44⁺ cancer stem cells with EpCAM^highCD44^- tumor cells in colon cancer by single-cell sequencing.

ABSTRACT: Cancer stem cells (CSCs) are considered to be responsible for tumorigenesis and cancer relapse. EpCAM^highCD44⁺ tumor cells are putative colorectal CSCs that express high levels of stem cell genes, while the EpCAM^highCD44^- population mostly contains differentiated tumor cells (DTCs). This study aims to determine whether single CSC (EpCAM^highCD44⁺) and DTC (EpCAM^highCD44^-) can be distinguished in terms of somatic copy number alterations (SCNAs). We applied fluorescence-activated cell sorting to isolate the CD45^-EpCAM^highCD44⁺ and CD45^-EpCAM^highCD44^- populations from two primary colon tumors, on which low-coverage single-cell whole-genome se

SUBMITTER: Liu M 

PROVIDER: S-EPMC6300344 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature