Dual monoclonal antibody-based sandwich ELISA for detection of in vitro packaged Ebola virus.

ABSTRACT:

Background

Rapid transmission and high mortality of Ebola virus disease (EVD) highlight a urgent need of large scale, convenient and effective measure for Ebola virus screening. Application of monoclonal antibodies (mAbs) are crucial for establishment of an enzyme-linked immunosorbent assay (ELISA) with high sensitivity and specificity.

Methods

The traditional cell fusion technique was used to generate a panel of hybridomas. Two mAbs were characterized by SDS-PAGE, Western blot, Indirect immunofluorescence assay (IFA). A sandwich ELISA was established using the two mAbs. The detection capability of the ELISA was evaluated.

Results

In the current study, we produced two murine-derived mAbs (designated as 6E3 and 3F21) towards Zaire Ebola virus glycoprotein (GP), the maj

SUBMITTER: Zai J 

PROVIDER: S-EPMC6300876 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature