Identification of pathogens in culture-negative infective endocarditis cases by metagenomic analysis.

ABSTRACT:

Background

Pathogens identification is critical for the proper diagnosis and precise treatment of infective endocarditis (IE). Although blood and valve cultures are the gold standard for IE pathogens detection, many cases are culture-negative, especially in patients who had received long-term antibiotic treatment, and precise diagnosis has therefore become a major challenge in the clinic. Metagenomic sequencing can provide both information on the pathogenic strain and the antibiotic susceptibility profile of patient samples without culturing, offering a powerful method to deal with culture-negative cases.

Methods

To assess the feasibility of a metagenomic approach to detect the causative pathogens in resected valves from IE patients, we employed both next-generation sequenci

SUBMITTER: Cheng J 

PROVIDER: S-EPMC6300891 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature