Clinical application and importance of one-step human CYP2C19 genotype detection.

ABSTRACT:

Background

To directly achieve cytochrome P450 2C19 gene ( CYP2C19) classification using one-step real-time fluorescent PCR detection and to verify the capabilities of this method with nucleic acid extracted from whole blood samples.

Methods

A human CYP2C19 genotyping kit based on one-step real-time fluorescent PCR detection was used to analyze whole blood or genomic DNA samples. This method was compared with pyrosequencing and another quantitative (q)PCR kit for its accuracy, repeatability, detection range analysis, sensitivity, specificity, and anti-interference analysis.

Results

The one-step real-time PCR method achieved a 100% accuracy rate compared with pyrosequencing and the other qPCR kit. When detecting different concentrations of known genes, concentrations o

SUBMITTER: Zheng LJ 

PROVIDER: S-EPMC6300956 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature