A novel primary culture method for high-purity satellite glial cells derived from rat dorsal root ganglion.

ABSTRACT: Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture method for satellite glial cells derived from rat dorsal root ganglia requires no digestion skill. Hence, the aim of the present study was to establish a novel primary culture method for satellite glial cells derived from dorsal root ganglia. Neonatal rat spine was collected and an incision made to expose the transverse protrusion and remove dorsal root ganglia. Dorsal root ganglia were freed from nerve fibers, connective tissue, and capsule membranes, then rinsed and transferred to 6-well plates, and cultured in a humidified 5% CO₂ incubator at 37°C. After 3 days in c

SUBMITTER: Wang XB 

PROVIDER: S-EPMC6301172 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature