Circ0043898 acts as a tumor inhibitor and performs regulatory effect on the inhibition of esophageal carcinoma.

ABSTRACT: Objective: The study aimed to investigate candidate circular RNAs (circRNAs) in regulating the pathogenic process of esophageal carcinoma. Methods: Specimens were collected from the patients with esophageal carcinoma. Total RNA was purified and treated with RNase R followed by RNA-seq in the purpose of screening the circRNAs in significant differentially expression. The expression level of the screened circRNAs were further validated using RT-PCR. The circular structure of the circRNA was validated with divergent and convergent primers. Overexpression vector was prepared in the purpose of raising the expression level of circ0043898 in the ECA-109 and Kyse-520 cells. The cell colony assay and MTS assay were conducted to determine the capacity of cell proliferation. Chamber ass

SUBMITTER: Wang W 

PROVIDER: S-EPMC6301825 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature