Mathematical-model-guided development of full-thickness epidermal equivalent.

ABSTRACT: Epidermal equivalents prepared with passaged keratinocytes are typically 10-20 μm thick, whereas intact human epidermis is up to 100 μm thick. Our established mathematical model of epidermal homeostasis predicted that the undulatory pattern of the papillary layer beneath the epidermis is a key determinant of epidermal thickness. Here, we tested this prediction by seeding human keratinocytes on polyester textiles with various fiber-structural patterns in culture dishes exposed to air, aiming to develop a more physiologically realistic epidermal model using passaged keratinocytes. Textile substrate with fiber thickness and inter-fiber distance matching the computer predictions afforded a three-dimensional epidermal-equivalent model with thick stratum corneum and intercellular lamellar lipid

SUBMITTER: Kumamoto J 

PROVIDER: S-EPMC6301960 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature