Aspartic Acid Residue 51 of SaeR Is Essential for Staphylococcus aureus Virulence.

ABSTRACT: Staphylococcus aureus is a common Gram-positive bacteria that is a major cause of human morbidity and mortality. The SaeR/S two-component sensory system of S. aureus is important for virulence gene transcription and pathogenesis. However, the influence of SaeR phosphorylation on virulence gene transcription is not clear. To determine the importance of potential SaeR phosphorylation sites for S. aureus virulence, we generated genomic alanine substitutions at conserved aspartic acid residues in the receiver domain of the SaeR response regulator in clinically significant S. aureus pulsed-field gel electrophoresis (PFGE) type USA300. Transcriptional analysis demonstrated a dramatic reduction in the transcript abundance of various toxins, adhesins, and immunomodulato

SUBMITTER: Nygaard TK 

PROVIDER: S-EPMC6302044 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature