Towards international standardization of immunoassays for Mullerian inhibiting substance/anti-Mullerian hormone.

ABSTRACT:

Research question

Is formulated and lyophilized, recombinant human Müllerian inhibiting substance, also known as anti-Müllerian hormone (AMH), suitable for the preparation of a WHO international standard to calibrate AMH immunoassays?

Design

The AMH content of a trial preparation, coded SS-581, was determined by five laboratories using seven immunoassay methods. Participants were requested to report the content of the preparation in terms of their method calibrators through the measurement of a minimum of five concentrations in the linear part of the dose-response curve. Participants were also asked to measure, concomitantly, a panel of six serum samples containing AMH at concentrations of 0.1-13.0 ng/ml.

Results

Across all assays, including two automated assays in de

SUBMITTER: Ferguson JM 

PROVIDER: S-EPMC6302068 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature