High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.

ABSTRACT: The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine fa

SUBMITTER: Kang B 

PROVIDER: S-EPMC6302096 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature