Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution.

ABSTRACT: Gold nanoparticles (AuNPs) have been used as a contrast agent for optical imaging of various single biomolecules. Because AuNPs have high scattering efficiency without photobleaching, biomolecular dynamics have been observed with nanometer localization precision and sub-millisecond time resolution. To understand the working principle of biomolecular motors in greater detail, further improvement of the localization precision and time resolution is necessary. Here, we investigated the lower limit of localization precision achievable with AuNPs and the fundamental law, which determines the localization precision. We first used objective-lens-type total internal reflection dark-field microscopy to obtain a scattering signal from an isolated AuNP. The localization precision was inversely propor

SUBMITTER: Ando J 

PROVIDER: S-EPMC6302141 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature