Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretion.

ABSTRACT: In many Gram-negative bacteria, the type 2 secretion system (T2SS) plays an important role in virulence because of its capacity to deliver a large amount of fully folded protein effectors to the extracellular milieu. Despite our knowledge of most T2SS components, the mechanisms underlying effector recruitment and secretion by the T2SS remain enigmatic. Using complementary biophysical and biochemical approaches, we identified here two direct interactions between the secreted effector CbpD and two components, XcpY_L and XcpZ_M, of the T2SS assembly platform (AP) in the opportunistic pathogen Pseudomonas aeruginosa Competition experiments indicated that CbpD binding to XcpY_L is XcpZ_M-dependent, suggesting sequential recruitment of the effector

SUBMITTER: Michel-Souzy S 

PROVIDER: S-EPMC6302157 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature